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KMID : 0545120100200040779
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 4 p.779 ~ p.781
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Heterotrophic bacterial growth on hoses in a neonatal water distribution system
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Buffet-Bataillon Sylvie
Bonnaure-Mallet Martine
Armelle de la Pintiere
Defawe Guy
Gautier-Lerestif Anne Lise
Fauveau Severine
Minet Jacques
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Abstract
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After preliminary tests indicated an increased number of heterotrophic bacteria, we investigated possible sources of contamination in a neonatal intensive care unit (NICU) water distribution system. Scanning electron microscopic examination of flexible metallic hoses associated with the system revealed the presence of a biofilm; partial 16S rDNA sequencing revealed that the biofilm contained Blastomonas natatoria. Purgation of the water system three times a day, reinforced faucet cleaning, decreasing the cold water temperature to 12C, and six repeated chlorinations at concentrations as high as 2 mg/L were not sufficient to eradicate the bacterial contamination. Replacing all of the rubber-interior flexible metallic hoses with teflon-lined hoses followed by heating the water to 70C successfully controlled the bacteria.
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KEYWORD
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heterotrophic plate counts, contamination, neonatal unit, biofilm, water distribution system
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